|
KMID : 1098420050130050288
|
|
Korean Journal of Medicinal Crop Science
2005 Volume.13 No. 5 p.288 ~ p.292
|
|
|
Molecular Cloning and Characterization of Type 2 Metallothionein cDNA from Codonopsis lanceolata (S. et Z.) Trautv
|
|
In Jun-Gyo
Lee Bum-Soo
Yi Tae-Ho
Yu Chang-Yeon
Yang Deok-Chun
|
|
|
Abstract
|
|
|
|
A class I type 2 metallothionein (CMet2) cDNA from taproot of Codonopsis lanceolata was isolated and characterized. A CMet2 cDNA was 572 nucleotides long and had an open reading frame of 234 bp with a deduced amino acid sequence of 78 residues (pI = 4.99). The deduced amino acid sequence of CMet2 matched to the previously reported type 2 metallothionein-like protein genes and showed 74% identity with that of G. max (BAD18377) and C. arietinum (CAA65009). Expression of CMet2 by the RT-PCR was increased at 1 hr after cadmium and hydrogen peroxide treatment, respectively.
|
|
|
KEYWORD
|
|
|
Abiotic stress, cadmium, Codonopsis lanceolata, metallothionein, RT-PCR
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|